Composite
nptII
Part:BBa_K2195002:Design
Designed by: Madeline Lee Group: iGEM17_WashU_StLouis (2017-10-27)
Neomycin Phosphotransferase (Codon Optimized for E. Coli) with medium promoter and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original part was redesigned to be compatible with sites 21 and 25 and was codon optimized for E. coli.
Source
See part page for BBa_K1021001 for more detailed information on source.